Results: A rapid, effective test enabling the screening of DNA alterations in the entire LDL receptor gene was developed.
The use of Basophile can reduce the size of the initial set of transitions, enabling fewer mass spectral experiments for the first iteration or enabling the screening of a broader collection of peptides in the same number of experiments.
Moreover, glycerol concentrations far lower than the previously described 16% can be used (Figs. 5), which is of great interest for new technologies enabling the screening of large panels of T-cell responses with a multitude of combinatorial-encoded MHC reagents (16).
The Biacore 4000 fluidics system allows the assignment of five detection spots to each of four flow cells, enabling the screening of four different compounds per cycle, where one of the targets per flow cell has to be assigned as a reference or preferably left blank.
We used the microscopic agglutination test (MAT) to test serum samples; the MAT was based on a panel of 15 Leptospira strains, enabling the screening of all recently reported serogroups for human and animal cases on neighboring Mayotte (2, 4, 9 ).
The present work describes the use of a technique to specifically assay very small quantities of enzymes, enabling the screening of a large number of recombinant enzymes from novel sources for their ability to enhance the digestion of plant cell walls by mixed rumen enzymes.
