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Mutagenesis is central to the study of microorganisms, enabling the functional role of specific genes to be determined (1, 2).
We develop a novel algorithm TAPAS enabling the functional neighbourhood of isoforms with high TS scores to be investigated.
Towards this end it is desirable to systematically establish a second genetic model system enabling the functional characterisation by mutant analysis.
FunCat is a hierarchically structured, scalable classification system enabling the functional assignment of proteins from any genome according to their physiological role, or metabolic pathway.
In contrast, high-throughput next-generation sequencing can provide abundant cDNA information by using long-read transcriptome sequencing by GS FLX Sequencer (454 Life Sciences/Roche, Branford, CT, USA), and subsequently protein sequences can be derived and used as references for proteomic mapping, enabling the functional profiling of protein diversity and quantification.
Here, we have set up a pipeline enabling the functional validation of a discovered mutation for any gene in a large breast cancer cohort by computationally connecting genotype to an extended, surrogate gene expression signature and utilizing this signature in gene chip datasets.
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Even if orthology is an evolutionary concept, rather than a functional one, it can be used to link functionally equivalent genes across genomes and enables the functional inference of unknown proteins using one or more functionally characterized orthologs in other species [ 6, 7].
For example, Gene Ontology and KEGG enable the functional interpretation of genes and gene products via enrichment analysis.
Associating cargo with these proteins, such as plasmid DNA, siRNA, or other proteins, can enable the functional delivery of these macromolecules into mammalian cells both in vitro and in vivo.
It outlines how recent developments in advanced materials, design methods, electronic control, and safety and legislative requirement have all enabled the functional requirements of modern automotive braking systems to be achieved in reliable and durable designs.
SPECT enables the functional visualization of MBs in the lungs 1 h post injection (A) and in the liver and kidneys 24 h post injection (B), while MRI enables the visualization of an anatomical image and SPION in the liver post injection and 24 h post injection (A and B).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com