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M002, an oncolytic HSV, has both copies of the γ134.5 gene deleted, enabling replication in tumor cells but thwarting infection of normal, postmitotic cells.
Sequence analysis showed that most PERV proviral DNA was significantly mutated, thus suggesting the inability to express functional viral RNA; however, it cannot be ruled out that compensatory recombination processes could occur enabling replication of defective proviruses.
Proteins such as latency-associated nuclear antigen (LANA) in KSHV have evolved to do this, for instance, by tethering the viral episome to host chromosomes during segregation and enabling replication of the viral episome by host replisome machinery.
The standard setup of vectors is a bi-functional system enabling replication in E. coli and maintenance in P. pastoris using as selection markers either auxotrophy markers (e.g., HIS4, MET2, ADE1, ARG4, URA3, URA5, GUT1) or genes conferring resistance to drugs such as Zeocin™, geneticin (G418) and blasticidin S.
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The panel said conclusions should be published, but not "experimental details and mutation data that would enable replication of the experiments".
The framework of bots, along with smart messages, enables replication of virtually any site or app onto the platform.
This knowledge transfer will enable replication of the WPCN CA model, where appropriate, in other jurisdictions.
This enables replication over large distances, but it may result in nodes containing inconsistent copies of data.
In many cases, digital technologies also can enable replication and distribution of products and services at close to zero marginal cost (Shapiro and Varian, 1999; Varian, 2000).
Finally, we make the tools used by our approach and (to enable replication) the experimental data and the results from their evaluation freely available on GitHub [12, 13].
The code, experimental data, and results are publicly available in our GitHub repositories at [12] for DBLModeller and [13] for MigSim to enable replication.
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