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The TaqMan Array Human microRNA Card Set v3.0 was used enabling accurate quantification of 754 human miRNAs.
Whereas Ki67 appears in all active phases of the cell cycle, BrdU staining allowed exclusive detection of cells in S-phase, thereby enabling accurate quantification of DNA synthesis.
The TaqMan Human MicroRNA A Array v2.0 contains 384 TaqMan miRNA assays enabling accurate quantification of 377 human miRNAs, six endogenous controls and one negative control.
The JAK2 exon 9 PCR was run along with each JAK2 V617F mutation-specific PCR, in order to normalise each sample for its input number of JAK2 templates, thus enabling accurate quantification of JAK2 mutant templates.
Here the reason for using collagen gels was that they trap cells, which die during PDT treatment, enabling accurate quantification of cell death; this is in contrast to monolayer cultures, in which the dead cells are more easily washed away, and can therefore be lost for quantification purposes.
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The simplex PCR assay enabled accurate quantification of the different alleles in the mix.
Recent advances in high sensitivity LC-MS/MS have enabled accurate quantification of low compound concentrations at tracer levels.
This is in contrast with the end point detection in conventional PCR, which does not enable accurate quantification of nucleic acids.
Even though the method for IR quantification is more laborious than gamma ray quantification, this new method enables accurate quantification of the probe in % ID/g without the use of radionuclides.
In particular, MAK2 enables accurate quantification of portable qPCR assays with limited sample throughput, where construction of a standard curve is impractical.
This experimental design enabled accurate quantification of antigen-specific CD8+ T cells by tetramer staining in mice vaccinated with H37Rv-OVA, ΔsecA2-OVA, ΔlysA-OVA, and ΔsecA2ΔlysA-OVA.
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