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The HOLO approach is broadly underpinned by the concept of nonlinear elimination, which enables segregation of the HO and LO components in ways that can effectively use heterogeneous architectures.
During metaphase I, attachment of spindle microtubules to the kinetochores enables segregation of dyads during the first meiotic division.
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Where available, DNAs from additional family members were sequenced to enable segregation analysis.
The unique barcodes and specific primers enable segregation of the pooled samples in NGS analysis later on.
Further in vivo experiments enabling segregation between direct versus indirect effects of absent blood flow will be required in order to answer this question.
These can be broken into two categories: direct maintenance of latency (e.g., enabling segregation of DNA into daughter cells) and immune evasion.
The availability of sequences for such genes will enable segregation tests to assess their involvement in pattern development and contribution to pattern variation within and between species (c.f. [ 19, 30]).
This chromatin model could explain how centromeres from multiple nonhomologous chromosomes, for instance, could cluster kinetochores and enable segregation by a smaller number of spindle microtubules, or how closely positioned adjacent genes could be independently regulated.
The current tourism practice at Machu Picchu in Peru mostly benefits the British train company Orient-Express, which operates PeruRail, and enables class segregation.
This includes Kmn1, which enables chromosome segregation during anaphase (Venkei et al., 2011), suggesting that mitosis could be disrupted.
Although the molecular mechanisms of MSC senescence after a limited number of cell divisions are still poorly understood, cell fusion processes which are known for MSC or asymmetric cell divisions may also contribute to this phenomenon which enables the segregation of daughter cells committed to either senescence or retaining reproductive capacity in correspondence to the parental cells [ 134].
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