Sentence examples for enables purification of from inspiring English sources

We describe a tandem RNA isolation procedure (TRIP) that enables purification of in vivo formed messenger ribonucleoprotein (mRNP) complexes.

This tag enables purification of recombinant protein by immobilized metal affinity chromatography (IMAC).

Frozen samples were quickly homogenized in QIAzol Lysis Reagent (Qiagen, Germany) and the <200 nt RNA fraction (including miRNAs) was immediately extracted using the RNeasy® Lipid Tissue Mini Kit (Qiagen, Germany) following the modified protocol which enables purification of miRNA and total RNA from the same sample in subsequent steps.

The HTB tag enables purification of Mad2p from denatured fission yeast extracts, in which protein phosphatases are inactive.

This arrangement of mobilities enables purification of, for example, nucleic acids from complex mixtures and excludes possible fouling species from the ITP zone.

The sensory epithelia was then thawed and total RNA was isolated, using the miRNeasy Mini Kit (Qiagen) without DNAse, which enables purification of total RNA of >18 nt.

Use of this TH-RFP reporter cell line enabled purification of mDA-like neurons from heterogeneous cultures with subsequent characterization of neuron transcriptional and epigenetic programs (global binding profiles of H3K27ac, H3K4me1, and 5-hydroxymethylcytosine [5hmC]) at four different stages of development.

Furthermore, the very low-level expression of readthrough proteins in the protoplast system does not enable purification of quantities sufficient for downstream analyses.

Immunoprecipitation using anti-FLAG resin enabled purification of sufficient protein to observe with SDS-PAGE analysis and subsequent colloidal Coomassie staining.

First, they have enabled purification of specific novel K+ channels such as the BK channel, a Ca2+ and voltage-gated K+ channel (Garcia et al., 1997).

The terminal 32 amino acids (a.a. 506 537), representing a hydrophobic domain that results in the formation of a glycosylphosphatidylinositol linkage to the membrane, was replaced by six histidine residues that enabled purification of the secreted protein with nickel-based affinity chromatography (Qiagen, Inc., Germantown, MD).