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The design of the Cre-dependent retroviral vector enables expression of encoded MOG and GFP genes only in association with Cre mediated DNA inversion.
Here in this study, we present an "ablate-and-replace" combination strategy that 1) destroys expression of the endogenous gene by CRISPR/Cas9 in a mutation-independent manner, and 2) enables expression of wild-type protein through exogenous cDNA.
Here, we have constructed a novel integration vector for mycobacteria that enables expression of genes from a cassette protected from transcriptional interference by bi-directional transcriptional terminators proven to be highly efficient in in vitro transcription termination assays.
The cDNA of P. blakesleeanus leuA was amplified and cloned into a plasmid vector that enables expression of genes in S. pombe strains.
Alternative splicing also enables expression of developmental stage-specific isoforms.
Alternative splicing enables expression of multiple messenger RNAs (4 ).
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However, this approach enables expressions of scientific uncertainty in more objective terms than possible at the time.
The system is also independent of the requirement for full-length cDNA clones to enable expression of genes and circumvents the need to clone and express individual splice-variants to assess functions of a gene.
The resulting suite of 288 yeast Gateway vectors is based upon the two commonly used GPD and GAL1 promoter expression systems that enable expression of ORFs, either constitutively or under galactose-inducible conditions.
Using Multisite Gateway five-DNA-fragment constructs vectors that enable expression of two tandemly situated cDNAs on a single plasmid were developed.
Further analysis determined that her antibody deficiency syndrome was due to a mutation in the CD81 gene, which did not enable expression of CD19 on the surface of the patient's B cells.
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