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The 6-Helix scaffold, which endowed the substituted thyrotropin receptor intracellular domain elements with spatial constraints analogous to those found in native receptors, enabled the reconstitution of a microdomain that consists of intracellular loops 2 and 3, and is capable of binding and activating Gα- s).
The thermophilic proteins show improved properties for structural and biochemical studies compared to their mesophilic counterparts, and purified ctNups enabled the reconstitution of the inner pore ring module that spans the width of the NPC from the anchoring membrane to the central transport channel.
These precursors enabled the reconstitution of the very last stage of the biosynthesis of peptidoglycan.
Gene silencing by targeting of the 3′UTR region of Prnp enabled the reconstitution of PrPc expression with a construct harbouring the Prnp open-reading frame.
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Other methods for crack growth curve reconstitution such as fractographic marking or textural fractography enable the reconstitution even if the striations are arduously detectable or wholly missing and also in the case of fractured bodies loaded with complex loading spectrum.
The amorphous saccharide excipients trehalose and inulin provided better stabilization than crystalline mannitol, and they enabled full reconstitution of the nanocomplexes.
In vitro reconstitution of previously uncharacterized shunt pathways enabled the identification of two distinct routes to a reduced shunt product including endogenous 'ene'-reduction and non-productive reduction by iridoid synthase when interfaced with endogenous alcohol dehydrogenases.
Therefore, the reconstitution of integrin into intact liposomes enabled us to further study their adhesion on different ECM proteins.
Finally, knockdown of these proteins in human cord blood-derived CD34+ cells enabled superior reconstitution of rag2 −/− γc −/− mice suggesting conserved functions of Bim and Bmf between humans and mice that may be exploited therapeutically to reduce HSCT-associated morbidity.
It enabled the incorporation of the Grand Trunk Pacific Railway GTPP).
This model most would involve the rapid recycling of activated receptors back to the plasma membrane thereby enabling rapid reconstitution of the pool membrane receptors to receive and process ongoing migration/chemotactic stimuli.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com