This originated the presence of a proto-splice site in the sequence of the nad7 gene, which, in turn, enabled the insertion of an intron at the same position in the different lineages.
The coding sequence for human NQO2 was amplified by PCR from a plasmid kindly supplied by Dr. David Jamieson (University of Newcastle-upon-Tyne, UK) using primers which enabled the insertion of the amplicon into pET46-Ek-LIC (Merck, Nottingham, UK) according to the manufacturer's instructions.
These mutants are highly heterogeneous in their insert lengths and sequences, indicating that the mutability of zeocin enables the insertion of a variety of target fragments with no discrimination.
Moreover, the critical design features to enable the insertion of ISEs into battery electrolytes are addressed.
A new module was developed to enable the insertion of object pose errors for testing the effects of perception inaccuracies on grasping.
This natural form of genetic modification has been adapted in laboratories over the last 20 years to enable the insertion of almost any foreign gene into plants [27].
Mobile group II introns, like retroviruses to which they are related, can also be used as integrating vectors, enabling the insertion of cargo genes at desired sites, with the advantage of high, controllable DNA target specificity [5], [9], [43].
Isoprenylation enables the insertion of these proteins into the cellular membrane, where they function in intracellular signal transduction pathways [ 17].
By October 1903, a design for an aluminum tube to enable the insertion of radium into a tumor had appeared in the Archives of the Roentgen Rays, the first British radiological journal.
The synthesized gene was flanked by compatible but non-regenerative restriction sites (BamHI and BglII) to enable the insertion of further synthetic genes for extension of the spidroin content.
Specific hydrolases, classified as muramidases, glucosaminidases, amidases, endopeptidases, and carboxypeptidases, are involved in breaking the covalent bonds of the existing PG sacculus, to enable the insertion of new material for cell growth and division (Scheffers and Pinho 2005).
