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Amplification of the two halves of PLCζ with these long primers enabled replacement of the PLCζ XYl residues 374 385 (KKRKRKMKIAMA) with the corresponding PLCδ1 XYl residues 480 491 (KPKEDKLKLVPE) to be achieved.
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That will enable replacement of non-renewable resources on which we depend on today with renewable sources and reduce GHG emissions and the footprint of agriculture.
Biologists hope the technique will enable replacement tissues to be generated from a patient's own cells for use against a wide variety of degenerative diseases.
The presence of transaminase activity is demonstrated in the cultured insect cell line Sf21 (Spodoptera frugiperda) at a level sufficient to enable replacement of rat liver in an undergraduate student experiment designed to demonstrate enzyme activity responsible for amino acid interconversions.
Accordingly, mechanisms must exist to enable replacement of isoforms while maintaining an almost crystalline regularity of structural pattern.
Therapies that enable replacement of β-cells are needed for both type 1 and type 2 diabetes, and we need animal models for identifying and testing these therapies.
Using plant oils in the chemical industry will not only enable replacement of fossil oil but will, at least equally importantly, enable substantial overall energy savings and generate added-value for agricultural products that cannot be captured by using them for energy production.
These "recombineering" ODNs were PAGE-purified (IDT, Leuven, Belgium) and contain 50-nt 5' homology arms corresponding to sequences directly flanking the single NcoI and HindIII sites of pBADcre [ 11] (Fig. 1A), respectively, and designed to enable replacement of the PBAD-driven cre gene in EL350 [ 11] with trfA.
This enabled mutagenised replacement DNAs to substitute the native gene and a screen to be performed to find ts mutants.
This enabled the replacement gfp cassette to be provided as a simple restriction-fragment excised from a suitable gfp-containing plasmid [ 10].
Introduced BglII and SalI restriction sites enabled the replacement of the GFP-cds of mAV4 [ 47] by the newly generated cds for GST.
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