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However, developments in high-throughput sequencing have enabled more extensive exploration of small RNAs.
The existence of an outgroup of nitrogenase homologs (termed Group IV) has been noted previously [ 27], but the current availability of genome sequences has enabled more extensive analysis.
More recently, the use of genome-based approaches has enabled more extensive investigations of sources of systematic bias, or inconsistency in phylogenetic analyses [ 102, 106- 108] and identified new effects difficult to detect in smaller data sets, such as site-specific changes in evolutionary rates among lineages, or heterotachy [ 109- 111].
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The main limitation of the evaluation was the lack of time and funding to enable more extensive community feedback and verification of the findings.
toxoMine complements the major community database ToxoDB by providing a data warehouse that enables more extensive integrative studies for T. gondii.
Indeed, the comprehensive phenotyping of mutants, which is a common practice in mice [ 4] for example, has begun in quail [ 5, 6], but more contributions are needed to enable more extensive comparisons between mammals and birds.
toxoMine complements the major community database ToxoDB by providing a data warehouse that enables more extensive integrative studies for T. gondii; therefore, we believe it will become an indispensable resource to the greater infectious disease research community.
While this method does not account for binucleation, as described by Walsh et al. (21), it has the advantage of enabling more extensive coexpression analyses, while still demonstrating relative proportions of cell types and changes in them, if not absolute numbers in individual hearts.
Appropriate financing, workable legal framework, and extensive support network enabled more than 200 homeowners' associations and owners of individual houses as well as 12 municipalities to implement various packages of energy efficiency measures in their buildings.
This enabled more technical discussion.
Here we describe how the use of gene structure analysis and comparative genomics enabled a more extensive understanding of these genes early in the discovery process.
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