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Although S. cerevisiae and P. pastoris share considerable genetic similarity that has enabled expression of similar genes and compatibility between vectors, P. pastoris has a strong preference for respiratory metabolism.
The advantage of RNA-Seq to measure gene expression over other methods, like microarrays, is that RNA-Seq is a probe independent platform and enabled expression profiling of all the glycosylation-related genes in bovine.
From a methodological perspective, in situ hybridization demonstrated less sensitivity to stress-induced changes in hippocampal M6a expression compared to quantitative RT-PCR analyses, but enabled expression to be quantified within specific neurons.
Evolutionary conservation of the prokaryotic gene expression machinery enabled expression of the six genes of the lux operon in chloroplasts yielding plants that are capable of autonomous light emission.
The high efficiency of homologous recombination in yeast, between the linearized plasmid and the PCR fragment, enabled expression of randomized cytoplasmic IncA domain chimeric proteins within yeast cells, permitting detection of those not binding to G3BP1 but still expressing EGFP via flourescence microscopy and colony-lift filter assay as described above.
AP and PH developed the vector that enabled expression and purification of AtGA3OX4.
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"It's a serious responsibility, and we work hard to strike the right balance between enabling expression while providing a safe and respectful experience.
"It's a serious responsibility, we work hard to strike the right balance between enabling expression while providing a safe and respectful experience".
The resulting suite of 288 yeast Gateway vectors is based upon the two commonly used GPD and GAL1 promoter expression systems that enable expression of ORFs, either constitutively or under galactose-inducible conditions.
Here in this study, we present an "ablate-and-replace" combination strategy that 1) destroys expression of the endogenous gene by CRISPR/Cas9 in a mutation-independent manner, and 2) enables expression of wild-type protein through exogenous cDNA.
The design of the Cre-dependent retroviral vector enables expression of encoded MOG and GFP genes only in association with Cre mediated DNA inversion.
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