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A microfluidic chip has been developed to enable the screening of chemicals for environmental toxicity.
The reported method will enable the screening of many chemically modified ppp-siRNAs, resulting in a novel bi-functional RNAi therapeutic platform.
High-throughput screening platforms with embryonic zebrafish now enable the screening of large chemical libraries for bioactivity providing opportunities for biological effects testing early in product development.
Here, we discuss these emerging platforms, which in some cases enable the screening of greater than 10 million enzyme variants, and highlight benefits and limitations of each technology.
In this article, we introduce a screening strategy that takes advantage of both the robustness of protein NMR spectroscopy as the detection method, and the basic principles of combinatorial chemistry to enable the screening of large libraries of fragments (>105 compounds) preassembled on a common backbone.
These approaches enable the screening of numerous substances for their impact on toxicity pathways.
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Results: A rapid, effective test enabling the screening of DNA alterations in the entire LDL receptor gene was developed.
Moreover, advances in the high-throughput measurement of denaturation curves and the application of selection methods to thermostability evolution have enabled the screening of larger libraries.
The method developed here miniaturizes existing HTS systems and enables the screening of a wide array of individual or combinatorial libraries in a reproducible and scalable manner.
The proposed system enables the screening of sulfonamides in honey samples with a low number of false positive results, with fast response therefore offers a new tool for consumer protection.
Use of plate-based methods increases experimental capacity about 10-fold over vial-based methods, which enables the screening of more parameters, with better resolution and higher statistical power.
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