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The new generation of DNA sequencers will enable sequencing of more than a dozen prokaryotic genomes in less than an hour, possibly making it cheaper and faster to sequence a whole genome than several genes for MLSA.
These advances will enable sequencing of entire global repositories of pathogens for future WGST analysis.
In principle, this approach could enable sequencing of modified bases in long reads (>10 kbp).
This protocol should stimulate and enable sequencing of other insect genomes.
The MiSeq data output per run (4.5-7.5 4.5-7.5l enable sequencingbof multiple samples via barcoding.
Pooling will also enable sequencing to move more readily into the diagnostic arena as a more cost effective method for screening multiple genes for rare functional variation.
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The short 3× Flag tag was knocked-in in this assay to enable sequence analysis covering the full length (left microhomology, knock-in fragment, and right microhomology) by next-generation sequencing (NGS) (Fig. 2a).
Engineering of catalytically inactivated Cas variants (nuclease-deficient or nuclease-deactivated [dCas]) combined with transcriptional repressors, activators, or epigenetic modifiers enable sequence-specific regulation of gene expression and chromatin state.
BLAST was integrated into the database to enable sequence similarity search.
The key molecules that enable sequence-specific gene activation to initiate embryonic development remain largely unknown.
CRISPR-associated (Cas) genes together with a single guide RNA (sgRNA) enable sequence-specific cleavage and editing of DNA.
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