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Five mm punch biopsies were treated overnight at 4°C with 0.3% dispase II (Roche, Almere, Netherlands) in PBS to enable separation of the dermis and epidermis, which were subsequently cultured separately in culture medium (IMDM with 10% NHS) in 24-well plates to allow spontaneous migration of T cells from the skin tissue fragments.
Such eigenmode expansions enable separation into ingoing and outgoing waves, i.e., incident, reflected and transmitted waves.
The S-probe contains a 5′ biotin moiety, a universal upstream primer sequence (black), a target hybridizing domain (red), and a target identifier domain (blue) to enable separation for subsequent measurement.
In 2007, the National Institute for Health and Clinical Excellence began introducing GRADE across its clinical guideline program to enable separation of judgments about the evidence quality from judgments about the strength of the recommendation.
To understand how this drives signaling, we have used advanced analytical methods that enable separation of effects on pathway-specific signaling from those that modify agonist affinity and mapped the functional consequence of receptor modification onto three-dimensional models of a receptor-ligand complex.
Briefly, thin sheets of skin were incubated overnight at 4°C in dispase (Roche) in PBS, to enable separation of epidermis and dermis.
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MVC is a classical pattern enabling separation of concerns (SoC) between the different aspects of projects.
Spectral unmixing enabled separation of the SRs and improved the contrast between the joint structures.
The dual-vector representation enables separation of kinematic function from mechanism topology.
This sealing technique enabled separation at temperatures up to 350 °C on a μGC column.
The mOSAIC ontology model enables separation of application-logic and cloud layers and consequently enhances cloud portability.
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CEO of Professional Science Editing for Scientists @ prosciediting.com