In the present study, GDNF was re-engineered to enable receptor-mediated transport across the BBB following fusion of GDNF to the heavy chain of a chimeric monoclonal antibody (MAb) against the mouse transferrin receptor (TfR), and this fusion protein is designated cTfRMAb-GDNF.
PET and SPECT have been proven to be particularly valuable for imaging targets in the central nervous system, enabling receptor occupancy measurements and dose selection for clinical candidates.
We have found that a proportion of NMDARs located in dendritic shafts, but not spines, are bound to glutamate under resting conditions, enabling receptor activation by bAPs.
To rigorously test the idea that Atg11 enables receptor-bound targets to activate Atg1, and thus drive local autophagosome formation, we developed a novel tool for monitoring Atg1 kinase activity in a test tube, and mixed cell extracts containing Atg1 with purified Atg11 and receptor-bound aggregates.
The Trojan horse enables receptor-mediated delivery of the fusion protein across the BBB so that the protein drug can enter the brain and exert the desired pharmacological effect.
A wide spectrum of functional peptides has been incorporated to different types of nanoparticles for the delivery of conventional drugs and nucleic acids, enabling receptor-specific cell binding and internalization, endosomal escape, cytosolic trafficking, nuclear targeting and DNA condensation.
Haubner and coworkers developed the first αvβ3-specific PET tracer [F]Galacto-RGD [ 8], a glycosylated cyclic pentapeptide, which demonstrated that PET with [F]Galacto-RGD enables receptor-specific monitoring of αvβ3 expression in murine tumour models.
U binds to transferrin, but conformational changes do not enable transferrin receptor recognition of the U-transferrin complex, ruling out this pathway for U to enter the cell.
Since the exposure of roach to fenitrothion was chronic, this may represent an adaptive cellular response to the continued inhibition of the androgen receptor, ultimately enabling androgen receptor-mediated activity even in the presence of the antiandrogenic effects of fenitrothion.
