Sentence examples for enable purification from inspiring English sources
In addition to supporting chromatography process development efforts (e.g., optimization screening), comparable product quality results at all scales makes this tool is an appropriate scale model to enable purification and product quality comparisons of HTPD bioreactors conditions.
Furthermore, the very low-level expression of readthrough proteins in the protoplast system does not enable purification of quantities sufficient for downstream analyses.
To enable purification from the media, the NA sequence was cloned downstream from a melittin signal peptide (MSP) sequence allowing recombinant proteins to be secreted from insect cells[18], [28].
A 6×His tag was added to each fusion protein to enable purification by affinity chromatography (see supplementary methods).
Using the program NGen (version 2) (DNASTAR , Inc, sequences within each tissue pool were combined and screened to remove poly(A) tails, SMART kit primer sequences (added during cDNA library construction), adaptor sequences (added to enable purification, amplification and sequencing by the FLX technology) and poor quality sequences (average sequence quality less than 14 in a 30 base window).
DGFc340 is a secreted α-DG deletion construct that contains only the minimal region of the α-DG mucin-like domain (aa 316 340), that is required for its functional glycosylation followed by a C-terminal fusion tag encoding the heavy-chain constant (Fc) moiety of human IgG1 (to enable purification of the secreted recombinant protein) (Hara et al., 2011).
We describe a tandem RNA isolation procedure (TRIP) that enables purification of in vivo formed messenger ribonucleoprotein (mRNP) complexes.
Recent advances in immunomagnetic cell sorting have enabled purification and isolation of pluripotent stem cells from marrow aspirates and have expanded stem cell technology to allogeneic cell sources.
Use of this TH-RFP reporter cell line enabled purification of mDA-like neurons from heterogeneous cultures with subsequent characterization of neuron transcriptional and epigenetic programs (global binding profiles of H3K27ac, H3K4me1, and 5-hydroxymethylcytosine [5hmC]) at four different stages of development.
The formation of N-protected secondary amide bonds bearing the N-p-methoxybenzyl N-p-methoxybenzyl N-p-methoxybenzyl intermediates in common orgroup solvensuredd enabled purificathen by chromatography; boiling trifluoroacetic acid removed the protecting group in the final solubilityhe synthesis.
The single fusion approach is also interesting because it enables purification to be combined with site specific labeling of the ELP with imaging agents, drugs, haptens or other moieties of interest.
