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In particular, we will discuss the use of engineered small molecules and natural proteins to enable pull-down, ChIP-Seq, ChIP-chip and fluorescence imaging of G-quadruplex structures in cellular DNA.
As part of an attempt to pin down the exact physiological role of these enzymes, both a N-terminal glutathione S-transferase and a C-terminal histidine-tagged form of the protein were created to enable "pull-down" assays and identify interacting partners which could aid in the functional definition.
[16] The analogues were biotinylated to enable pull-down of the cross-linked complex out of the cell lysates.
Antibodies used for immunoprecipitations were either directed against H4, which is universally present in all nucleosomes, or against an epitope shared between H3 and H3.3, which enabled pull-down of all nucleosomes with the exception of centromeric nucleosomes that contain the variant histone CenH3.
CEO Drew Houston told reporters the company has no plans to charge developers, after announcing the launch of two new APIs to enable pulling files down from the cloud and syncing.
It had well developed flexors and extensors in its forearms, which enabled it to pull down and securely hold down large prey so it could deliver a killing bite without endangering the vulnerable elongate canines.
Pull down help is therefore included.
Sag or pull down is related to thick reservoirs.
In vitro pull down assays.
This will pull down a text box.
Conversely, HA-ERK1 could also pull down Flag-FBW7.
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