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Substrates were internally labeled by 3'-filling to enable assays of 5'-3' exonucleolytic digestion initiating opposite the structured end (Fig. 5A).
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Cell death was assessed by PARP cleavage assay, as we have previously described (Collett and Campbell, 2006), and by flow cytometry, using PI for nuclear staining, to enable assay of the hypodiploid sub-G0/G1 peak (PI-labelled apoptosis; Riccardi and Nicoletti, 2006).
EST-SSRs constitute a novel source of markers that are physically associated with coding regions of the genome, and this can enhance the role of genetic markers in germplasm evaluation by enabling assay of the variation in transcribed sequences and genes where function is known.
Advanced molecular methods moreover enabled systematic assays of the patterns of pleiotropy on an array of organisms and phenotypes.
High-density assays for SNP detection have recently been developed and one such platform from Illumina enables simultaneous assays of 384 markers from a single DNA sample.
A method for separation of Mo from Zr, Nb, and other major elements of rubble samples from the Fukushima Daiichi Nuclear Power Station (FDNPS) was developed to enable 93Mo assay of the rubble samples.
Our work confirmed that this mutant provided an excellent negative background, enabling the assay of peptide-dependent QS activation without interference by endogenous CSP.
Multi-layer assays enable the analysis of 3D migration of cells between layers of paper that contain different types of cells.
Such antibody-activated reporters could someday enable point-of-care serological assays for the rapid detection of infectious diseases.
These assays enable studies of quantitative trait variation for a large number of fitness-related traits.
To enable comparison of assays for each laboratory, the geometric mean titer (GMT) was calculated across runs and replicates to give a single value for each sample for each MN assay method.
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