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ALI is an EMS level mission concept which would allow autonomous in situ exploration of the lunar poles within the next decade.
Despite a clear reduction in seed number at the low EMS concentration, no effect was observed on M2 seed viability until the 0.3% EMS level.
However, the huge variation in seed viability observed at the 0.3% EMS level, suggests that a high mutation frequency has been reached at this concentration.
10 In both communities, the EMS level is intermediate where the highest qualified emergency medical technicians can give CPR with AED, perform advanced airway management and inject intravenous fluid or drug.
Considering how other recent studies on the effects of heat on mortality and hospitalizations in King County do not report increased risks for these age groups, our findings indicate that the effects of heat on child health may be captured at an EMS level, but not higher up in the public health system [ 50].
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We use confocal and multiphoton microscopy for live imaging and physiological recording in tissue slices, high resolution fluorescence and light microscopy, as well as 3D reconstructions at both the light and EM level.
We performed TUNEL at the electron microscopic (EM) level, which permitted correlation of DNA fragmentation with chromatin condensation.
At the EM level, nuclear envelope ruptures were not apparent as they had been in imaginal disc nuclei.
With a straight forward re-localization of the ROI at the EM level, serial immuno-EM and EM tomography of membrane tubules induced by amphiphysin 2 overexpression was successfully performed on cells previously acquired by live LM.
However, no effect could be seen on the perinuclear localization of the Golgi marker, and also studies with flotillin-depleted cells revealed no detectable morphological changes at the EM level (data not shown).
Importantly, even if these structures look normal at the EM level, it does not mean they are fully functional.
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