First, cryo-EM analysis and lysozyme treatment clearly showed that the spinae are not extensions of the cell wall, hence belonging to the family of non-prosthecate appendages.
Recently, graphene attracted a great deal of attention of EM analysis and characterization [7, 8].
Only a minority of enterocytes might display the hallmarks at the time of EM analysis, and the histological presentation of MVID varies from patient to patient.
We thank Alice Liang for EM analysis and Peter Lopez for cell sorting.
In order to identify candidate RER whorl forming proteins in Ae aegypti midgut epithelial cells, we took advantage of the fact that an amino acid meal is sufficient to induce whorl unwinding, which simplifies both EM analysis and protein identification by mass spectrometry because it eliminates interference from abundant blood meal proteins.
Taken together, the EM analysis and mutagenesis data argue strongly against a specific interaction between the linker and AAA4.
Protease protection assays, EM analysis, and analysis of the effects of lysosomal inhibitors should be considered to strengthen this aspect of the study.
Thin (600 800 Å) sections were collected on copper 75/300 mesh grids for conventional EM analysis and stained with 2% (w/v) uranyl acetate and Reynolds' lead citrate.
Electron microscopy (EM) analysis and immunolabeling for SERPINA1 on liver thin sections showed reduced and smaller SERPINA1-positive inclusions in the cytoplasm of mice injected with HDAd-TFEB.
Conception and design: HAO, SB, BG, EM ; Analysis and interpretation: HAO, MA, AG, JLB, EM, BG ; Drafting the manuscript for important intellectual content: HAO, MA, AG, JLB, DM, NB, GO, EM, BG.
