Our previous microarray analyses have revealed that the transcript profiles of control plants transformed with the empty pBin61 transformation vector are approximately equal to those of the wild type healthy plants [ 35], and therefore only the wild type plants were used here as controls.
Leaf cells isolated from non-transgenic (NT) Nicotiana benthamiana plants, transgenic plants expressing Aox1a or Aox-E sequences under the control of the cauliflower mosaic virus 35S promoter, plants harboring a 35S MtRDR1 transgene super-transformed with the 35S: Aox1a transgene, or from plants transformed with an 'empty' transformation vector were placed in an oxygen electrode.
Dr. Rivera Colón published "Between the Runway & the Empty Tomb: Bodily Transformation and Christian Praxis in New York City's House Ball Community" in an edited volume by Dr. Samuel Cruz entitled Christianity and Culture in the City: A Postcolonial Approach.
Compared to empty vector (EV; transformation control) plants, a significant reduction was observed in NaMYC2 transcript accumulation in MYC2-VIGS plants before (ANOVA, F1,6=339.22, P=0.0001) or 1 h (ANOVA, F1,8=418.72, P=0.0001) or 3 h (ANOVA, F1,3=42.41, P=0.007) after WOS induction.
Instead, Moss and Frith shared a time-lapse video, showing the property's transformation from empty and barren to staged and seemingly move-in ready.
"Excavations in the 1920s and 1950s uncovered large parts including the gatehouse, the moat, some interior buildings and perhaps part of the dungeon, so it's clear there's significant archaeology here". The Castlegate redevelopment will also see a new green "corridor", the transformation of empty shops and work to protect the city's Old Town Hall.
The addition of cycloheximide prevents transformation of empty plasmids, which remain circular due to incomplete ClaI and SalI restriction digest, as those vectors still maintain the CYH2 gene that confers dominant sensitivity to cycloheximide.
These products were ligated together with KpnI/NheI digested PGL3 empty vector followed by transformation.
Transformation of empty vector into a ctk1Δ strain produced no change in TBP cross-linking, which was still seen in the PMA1 coding region.
