Closets, Ms. Dazzo explained, should be partially emptied to create the sense that there's plenty of storage space.
DNA sequences from the human miRNA-155 host gene (mir155hg) (GeneID 114614) were amplified by PCR with primers Odp.1572/Odp.1573 and cDNA generated from human PBMC and cloned into vector pCMV-Empty to create the plasmid pMIR155HG.
Bring an empty jar to create a garden butterfly feeder.
More often, immigrants simply want to use America's empty space to create a better life.
Now the lethargic ticket sales, leaving stadiums empty enough to create echoes, have put them on the defensive again.
He uses a stationary camera, deployed at odd angles in vast, empty rooms, to create an atmosphere of palpable dread.
"Once MASS MoCA commissioned me, I was granted a few months to be able to spend time alone in the empty building to create these on-site studies," Prey explains.
This value was then divided by the average of the empty spots to create a signal-to-noise ratio.
To generate GST fusion constructs, PCR products containing the full-length human CLU, or the alpha and beta chains were digested and ligated into pGEX4T-1 (GST) empty vectors to create GST fusion protein expression vectors pGEX4T-1 CLUU, pGEX4T-1 alpha, pGEX4T-1 beta). pGEX4T-1 beta
