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The blue curve refers to an empty sample and the red curve to a eumelanin-impregnated PSi layer.
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The empty sample and reference pans were taken of equal mass for analysis.
Since the slopes of the linear fits related to the impregnated sample are always higher than those related to the empty sample, the results of Figure 4 demonstrate that the refractive index of the impregnated PSi layer is higher than that of the empty PSi layer in the whole spectral range examined.
CI was obtained from the relationship between the height of the crystalline peak for cellulose (I002) and the height of the minimum (IAM) between the 002 and the 101 peaks, after background subtraction, according to Equation 1. (1) CI % = I 002 – I AM / I 002 × 100 The background signal was obtained measuring the empty sample holder under the same conditions used for the samples.
By analyzing empty sample containers, varying the amount of sample, and flushing out samples before analysis, the SAM team has concluded that the contaminant now accounts for only 1% to 3% of the carbon appearing as carbon dioxide.
The background subtraction was carried out considering the spectrum obtained for an empty sample holder in the same 2 θ range.
CI was obtained from the relationship between the intensity of the 002 peak for cellulose I (I002) and the minimum dip (Iam) between the 002 and the 101 peaks according to Equation 1: (1) C I = I 002 - I a m I 002 × 100 The background subtraction was carried out considering the spectrum obtained for an empty sample holder in the same 2θ range.
where Cp is the specific heat capacity, Δq is the heat flow difference between the sample and the empty pan, and m is the mass.
We used RELION (version 1.3-beta) for automated selection of 126,727 particles from 1310 micrographs for the empty Pf80S sample; and 158,212 particles from 1081 micrographs for the Pf80S emetine sample.
Furthermore, counts for these six genes were similar to those found in empty samples (Table 1) and remained undetectable in the 3- and 5-oocyte samples (Supplemental Table 1).
The signal intensity of the respective band was estimated with the "volume rectangular tool" and corrected versus the background of a band of equal height and size (area) in an empty sample lane.
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