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Mock, cotransfected with reporter construct and an empty effector construct; control, cotransfected with effector construct and an empty reporter construct (set to 1).
Consistently, the most proximal 31 bp showed an unspecific activation, similar to that induced by the empty reporter vector.
Second, a negative control was obtained by protoplast transformation with the empty reporter vector and effector vector.
An empty reporter vector, lacking a cloned target site in the 3' UTR, was not affected by miR-145 overexpression.
The inserted AQP5 promoter was functional given that the promoter-less (empty) reporter vector (pGL3-basic) showed significantly less luciferase activity.
Knockdown was assessed by calculating luciferase signal ratios for specific miRNA/non-targeting control, using empty reporter vector as control for non-specific effects.
Similar(50)
The empty luciferase reporter construct (left panel) or the pMIR reporter containing the wild-type ZEB2 3′ UTR (right panel) was coexpressed with vectors for ebv-miR-BART7 or ebv-miR-BART22.
"They put that up there whenever the stage is empty," a reporter from The Nation told me, helpfully.
In the latest Drinking & Talking, our panelists relive their favorite moments -- and empty their reporter notebooks -- from this primary season.
Values shown by the fluc to rluc ratio were normalized to an empty luciferase reporter control.
None of the miR-142 constructs had an effect on the empty pMIR reporter vector (Fig. 2A, left panel).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com