Exact(3)
(B ) Growth of ten-fold serial dilutions of SPC110 shuffle strains carrying the empty integration vector (null) or SPC110 (WT) or SPC110 mutant alleles on the integration vector.
Gene knockout was performed as previously described., When necessary, empty integration plasmids were transformed into appropriate yeast strains to ensure that strains being compared have the same auxotrophic genotype.
For protein-coding genes, gene expression profiles were compared by DNA microarray analysis between the N-His-AdpA-producing Δ adpA strain and the Δ adpA strain containing the empty integration vector pTYM19 on the chromosome.
Similar(57)
Additionally, SE02a was constructed as a control by integrating the empty vector, pSA, into SE02 at neutral integration site II (NSII).
The analyses of paralogous and orthologous empty sites indicated that the integration sites of Spy transposons are precisely between the host AAA and TTT nucleotides, without deletion and duplication of host sequences upon Spy insertion.
Gastric emptying is determined by the integration of motor activity of the stomach and upper small intestine, controlled by electrical slow waves generated by the interstitial cells of Cajal (4, 13).
Alu insertion loci were included in phylogenetic analysis if amplicons were generated in at least seven out of the 11 cercopithecine taxa in our panel, and only 2 distinct classes of amplicons were obtained (i.e. Alu filled size and pre-integration or Alu empty size).
Indeed, David Cameron's foreign policy pronouncements appear too often to have swung between two poles: between hasty and empty grandstanding, over the EU financial integration and Syria, and seeking to dodge difficult issues, including Sri Lanka over human rights and China and Tibet.
A law passed this summer essentially allows empty military facilities to be transformed into "integration zones".
This isolation cell is one of many and the first of many more to come, since a law was passed this summer that will essentially allow empty military facilities to be transformed into "integration zones".
The economic integration he envisioned would make nations an empty exercise in symbolism, since all important decisions would be beyond the purview of national authorities.
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