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Put a lift strip in the empty insert.
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TEER values are normalized to the TEER of empty inserts.
Values are normalized to the TEER of empty inserts.
TEER was measured after 1.5 h after stimulation and is normalized to empty inserts.
TEER across the filter was monitored over time and is normalized to empty inserts.
a TEER was measured 1.5 h after stimulation and is normalized to empty inserts.
TEER across the filter was measured 1.5 h after HBP stimulation and is normalized to empty inserts.
Control RPE cultures were incubated with empty inserts without cultured microglia.
Of about 5490000 reads obtained by the high-troughput sequencing, 97% were clearly attributable to the corresponding bar-coded samples (42% for the PC-C40 and 56% for GDS6 samples, respectively) and adopted a profile with two prominent 21- and 24-nt peaks (excluding the files with inserts without adapters and empty inserts) (Table S1 and Fig. S1).
Empty inserts served as control.
All measurements were corrected for resistance of culture medium and empty inserts.
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