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The amplified fragment was inserted into the empty Gateway vector pGWB1 (Nakagawa et al., 2007).
Following recombination, clones can also contain empty Gateway donor vector in which the toxic ccdB gene, which normally prevents growth of the empty vector, is no longer functional due to mutation [12], [14].
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For control cells, empty vectors without Gateway recombination cassettes were used.
We stopped in Salalah, Oman's second-largest city and gateway to the Empty Quarter, to meet Ali, a guide and driver.
THE views from the empty upper floors in One Gateway are also something that might be described as heavenly -- both uptown and downtown Manhattan are clearly visible across the Passaic and the Hudson Rivers, as well as the gold dome of Newark's City Hall, the nearby hills of Montclair and the church spires of Orange.
Pristine and mostly empty, Niihau has been a perfect gateway for Hawaiian monk seals as they have recolonized their species' ancestral habitat.
Here in Dawson County, a 2,358 square-mile expanse of empty plains and badlands that was once called the gateway to Montana, there were 138 rural schools in 1919, according to a fading chronicle of their history.
On Monday, after a concession by the protesters, airlines were flying dozens of empty planes out of the besieged international airport here, Thailand's main tourist gateway.
Located where the Hudson and East rivers empty into one of the world's premier harbours, New York is both the gateway to the North American continent and its preferred exit to the oceans of the globe.
When Node A is turned on with empty ARP cache and long-term table, if the neighbor nodes that know the true MAC address of the gateway router provide that information to Node A, then Node A can make a correct decision on the MAC address of the gateway router based on the majority of the votes.
Brokers:Gateway Arms; Citizens Properties.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com