Sentence examples for empty fret from inspiring English sources

Time-resolved responses of ABAleon2.1 in (A – C ) the hypocotyl and (D – F ) the root maturation zone, and of (G – I ) the empty FRET cassette in the hypocotyl.

DOI: http://dx.doi.org/10.7554/eLife.01739.007 10.7554/eLiFigure3 figureigure 3—figure supplement 1. ABAleon2.1 but not the empty FRET cassette responds specifically to ABA.

Recombinant empty FRET control (F3) and all ABAleon versions were purified and biochemical characteristics of these proteins are provided in Table 1.

(E ) anti-GFP immuno-detection and (F ) PageBlue stain of 1 µg empty FRET cassette (F3) and ABAleon proteins after purification.

Absorbance at 434 nm (mTurquoise) was used to calculate concentrations of the empty FRET and ABAleon proteins (Goedhart et al., 2010) and the ratio of Abs515 (cpVenus173) and Abs434 was used to estimate protein purity.

pET-F3_II (empty FRET) and pET-ABAleon_II versions in BL21-CodonPlus (DE3 -RIL cells (Stratagene) were grown at 37 °C in 2 L Luria Broth (LB) meDE3 -RILtaining 25 µg/mL Kanamycells

In control seedlings expressing only the empty FRET-cassette no response to ABA was detected.

Finally, ABAleon2.1, ABAleon2.1 mutants and the empty FRET-cassette were sub-cloned from pUC-ABAleon2.1, pUC-ABAleon2.1x_II and pUC-F3 plasmids into the barII-UT and hygII-UT plasmids to obtain the barII-UT-ABAleon2.1, barII-UTF3 and hygII-UT-ABAleon2.1 and mutant plasmids for expression in plants.

I knew that middle age was dowdy and maudlin and complaining, that our hormones ride rollercoasters; that we thrill to adulterous affairs; that in midlife we lose parents and sometimes friends and feel as if our identities are dissolving – it's why we cry over empty nests, fret over our truncated futures, suffer breakdowns and breakups and mourn our disappearing youth.

Pessimists envisaged a repeat of Atlanta 1996, when events took place in front of rows of empty seats, fretting about the impact a poor response from the public might have on the next generation of Paralympians.

He left empty-handed, fretting about losing two items that were so close to his heart.