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To assess the relevance of this phosphorescence background signal from the catheters for BLI in vivo, catheters containing biofilms of ACTgLuc-expressing C. albicans and empty catheter pieces were implanted subcutaneously.
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Next, we opened the door and added CTZ to the catheters and compared the specific BLI signal from biofilms of ACTgLuc-expressing cells with light emanating from empty catheters.
Unlike for the empty catheters, the BLI signal intensity from catheters containing ACTgLuc-expressing cells increased significantly above the phosphorescence signal upon CTZ addition (Fig. 1 panels B and C).
Feeling less tired and looking better than I'd expected, I change my pads and empty the catheter myself, passing time by watching Georges Méliès films and Paul Merton's programme on Buster Keaton on my laptop.
A scalpel was used to make a skin incision and a tunnel was created under the skin by blunt dissection with hemostats to accept the empty Word catheter [see Figure 1, Panel B].
Foos watched as the wife tried to help her husband out of his wheelchair and emptied his catheter bag.
The latter includes a wide range of activities from emptying a catheter bag, safety or security checks, to preparing meals or drinks.
I hoisted the gentleman out of bed, assisted with a shower, gave him a shave, emptied his catheter bag, helped with medication and breakfast – all in a thoughtful and caring way.
Hannah J Davies "I'm just going to empty your catheters: who wants to go first?" It's the concluding part of this wonderfully tough comedy, as Kim confronts outsourced care providers Buccaneer 2000, along with a familiar old face from the hospital.
At all time points, we detected a clearly visible signal from empty implanted Arrow catheters without addition of CTZ (Fig. 1 panels B and C).
The urinary bladder was catheterized using a 10-French silicone catheter, emptied and then filled with sterile saline solution (approximately 8 mL/kg of body weight).
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