Sentence examples for empty backbone from inspiring English sources

Two naïve control sera did not detect the fusion proteins and a control fusion protein (pPeptide; empty backbone of fusion protein without mimotope insert) was not detected by any of the rhesus monkey sera.

The empty backbone vectors (pCDH or pGIPZ) were used as negative controls.

The empty plasmid backbone is referred to as pKT2-HF-U6-shRNA-GFP. Later these plasmids were digested with HindIII to remove the CMV-eGFP expression cassette to make pKT2-HF-U6-shRNA.

All selections resulted in colonies growing on concentrations of antibiotic 1 2 fold greater than the concentration at which the PAO2003 or the PAO2003 strain containing the empty vector backbone were able to grow, indicating that genomic insert contained within surviving library clones were providing a moderate selective advantage.

To avoid the empty vector backbone influence, we applied the pRNAT-MN as control cells instead of Miapaca-2 cells.

Then, cells were transfected using lentivirus containing either the empty pMIRNA backbone vector (control) or pMIRNA vector with mir-524-5p mir-524-5p mir-524-5pes (premature from System Biosciences).

The shRNAs targeting EDD were inserted into the empty vector backbone (sequences: 5′-TGACAGCAGAACAACATAATT-3′ in pSRP; 5′-GCTCGTCTTGATCTACTTTAT-3′ in pLKO.1) (courtesy of K.P. Lu).

In addition, hMSCs were transfected with mir-524-5p mir-524-5p mir-524-5ppty pMIRNA backbone (PM_40) vector lentivirus (andnegatheemptytrol) and, subsequently, cells were allowed to differentiate for 14 days pMIRNARNA qPCR analysis.

Instead, it consistently resulted in a small but reproducibly and statistically significant increase in cell proliferation of Hs578T, MDA-MB231, and T47D cells stably transduced with MLV-HA-RASSF1C (1comparedred to an empty MLV-backbone (BB) as demonstrated by H-thymidine cell proliferation assays.

Empty pT3TS plasmid backbone was obtained by digesting pT3TS-SB11 with BglII [40].

For lentiviral-mediated knockdown of Trp53, we generated a vector (pLenti X1 Puro DEST, Addgene 17297) containing the U6 promoter (derived from pENTR/pSM2 (U6), Addgene 17387) driving expression of a previously described (Dickins et al, 2005) miR30 format shRNA against Trp53 (1224) or expressing an empty (ns) miR30 backbone.