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When they are copied into an empty allele, they are still retained by the donor allele.
During DNA repair, the parasitic element is copied into the previously empty allele.
Segregation of the empty allele (without any requirement for deletion) would also explain the frequent occurrence of species lacking the intein.
Segregation of the empty allele (without any requirement for deletion) would also explain the frequent occurrence of closely related species that lack the intein.
To secure the long term stability of the HEG, any reduction in the susceptibility of the empty allele for homing must be accompanied by a large enough reduction in the fitness of the empty allele (h' < h ⇒ fitness of X = 1-r' where r' > 0).
In a diploid cell heterozygous for the intein, cleavage of the empty allele by the homing endonuclease will be followed by DNA repair performed by the host repair machinery, using the occupied allele as a template [ 8].
Similar(53)
To determine whether Gn or Gc is involved in N packaging, we transfected cells with all viral components or equivalent amounts of plasmid encoding the GcW1 allele or empty vector.
Transgenic lines were generated by transformation of the mutant plants with a Sor1 (wild-type) allele or control empty vector.
After transfection with either empty vector or the wild‐type allele, MitoTracker staining revealed a filamentous network.
COS7 cells were transfected either with each allele and with the empty vector.
Hairy roots a stable transgenic L. japonicus Gifu reporter line homozygous for the T90 reporter fusion and the nfr1-1 mutallelelele transformed with the empty vector (EV), pUB NFR1-mOrange (NFR1 ), pUB NFR1-mOrange (NFR1 ), or pUB NFR5-mOrangee (SYMRK ) were generated.
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