Exact(1)
All raw reads were pair-end sequenced and subjected to the following quality control to produce clean reads: (1) raw reads including adapter sequences and empty adapter were discarded; (2) reads including unknown N bases comprising more than 3%% of the total length were filtered; (3) reads including low-quality bases that comprise more than 15%% of total length were discarded.
Similar(59)
After excluding low-quality reads such as empty adapters, 26 million clean reads were generated in each pool.
Low quality bases, empty reads, and adapter sequences were removed using CLC-BIO Genomic Workbench, version 5.1 (CLC-BIO).
Low-quality bases, empty reads, and adapter sequences were removed using CLC-BIO Genomic Workbench, version 5.1 (CLC-BIO) and ERNE-FILTER (Del Fabbro et al. 2013).
Raw reads were cleaned by removing adapter sequences, empty reads, and low quality sequences.
Sequences from the Illumia sequencing were deposited in the GenBank Short Read Archive (Accession number: SRA055079).The raw reads were cleaned by removing adapter sequences, empty reads, and low quality sequences (reads with unknown base pairs 'N').
After filtering 3′ adapter sequence, empty reads, low-complexity reads, and low-quality reads, a total of 4,847,568 and 4,883,691 21 bp length clean tags were obtained that corresponded to 446,284 and 423,464 distinct tags for Yacheng 05 179 before and after S. scitaminea inoculation, respectively (Table 3).
Attach an empty syringe toe the adapter at the free end of the tube.
Of about 5490000 reads obtained by the high-troughput sequencing, 97% were clearly attributable to the corresponding bar-coded samples (42% for the PC-C40 and 56% for GDS6 samples, respectively) and adopted a profile with two prominent 21- and 24-nt peaks (excluding the files with inserts without adapters and empty inserts) (Table S1 and Fig. S1).
After removing artefacts (products of multiple adapter ligations or empty constructs), 244,994 sequences remained for analysis.
Ensures Infra scan list is not empty while connected to a wireless display adapter.
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