Sentence examples for employs restriction from inspiring English sources

The currently widely used Genome Walker Kit from Clontech employs restriction enzyme cleavage of DNA and adaptor-ligated genomic DNA construction, and also involves a nested PCR strategy.

We can get fragment size information by employing restriction enzymes.

A number of these approaches employ restriction endonucleases to discriminate between sequences.

Historically, widely applied methods for the detection of DNA polymorphisms employed restriction enzymes and were limited to detection of changes in the restriction sites.

This study employed restriction enzyme digestion in conjunction with the recombinant cloning of a Norway rat Vegfr2 gene restriction fragment into the eukaryotic expression vector pCMV6.

We employed restriction digestion and S1 nuclease treatment in conjunction with PFGE and Southern Blot analysis to confirm the sizes and topologies of the plasmids present in JCM1046.

A synergistic approach of combining libraries created by different methods will help in reducing gaps in the physical map that may result from uneven distribution of restriction sites of the employed restriction endonucleases.

It employs two restriction enzymes; HpaII, methylation-sensitive and MspI methylation-insensitive (isoschizomer), to digest the genomic DNA, contrast the digestion products generated by these enzymes and finally use ligation-mediated PCR (size range is limited from 200-2000 bp) (Khulan et al., 2006).

This method employs two restriction enzymes to generate short fragments in the analysis of AFLP.

Using an established model of developmental programming that employs protein restriction of maternal diets during gestation or lactation,, we find that although changes in expression level of imprinted genes can be detected in liver of offspring from dietary-restricted female mice, DMR methylation appears to be robust.

The first zoning policy employed no restriction while the second conversion restriction zoning policy restricted urban-agricultural expansion in the Guwahati Municipal Development Authority (GMDA) proposed green belt, extending to a third zoning policy providing wetland restoration in the proposed green belt.