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To reduce the number of false indels arising from wrong alignments, we employed sequence alignment programs based on structures to perform pairwise alignments of non-redundant structures in each family.
We have used profile alignment to avoid the challenges of multiple sequence alignment on large families.
In order to minimize problem (3), we employed a sequence alignment based on structure rather than a sequence alignment based on sequence.
Degenerate primers based on conserved regions were designed by employing multiple sequence alignment strategy using ClustalW.
By employing multispecies sequence alignment we identified an ancient (tetrapod-teleost conserved) non-coding architecture within the introns of GLI3.
Using STAP, individual query sequences are analyzed by a three-step process, with each step employing both sequence alignment and phylogenetic analysis.
If activated, ReXSpecies 2.0 can employ several sequence alignment tools to redo the alignment, per default muscle [ 61] is used.
We employed the sequence alignment functions available as part of the Bioinformatics toolbox in MATLAB to create the folds.
Subsequently, MEGA version 6.06 was also employed for multiple sequence alignment of the amino acids.
Katoh, K. MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform.
As a result, other supermatrix methods sidestep multiple sequence alignment by employing clustering techniques to determine "alignable regions".
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