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However, conventional SEM requires dehydration and metal coating of biological samples before observation, and because biofilms consist mainly of water, sample dehydration may influence the biofilm structure.
To enclose the stained tissue sample, dehydration and mounting processes were conducted after separating the MMIHC device from the slide.
Caution was taken to avoid sample dehydration.
Following sample dehydration through graded alcohol concentrations (50%, 75 %, 96 % and 100, the aorta tissue was embedded into paraffin.
This qualitative study employed purposive sampling.
We employed stratified sampling for this study.
The study had employed multistage sampling technique.
Qualitative interview study, employing extreme-case sampling.
All other investigations completed on participants with a VO2 max less than 50.0 mL kg−1 min−1, i.e. untrained or physically active, employed passive methods of dehydration or allocated ~2 h recovery post-active dehydration [41, 42].
To dehydrate the samples, a dehydration chain of increasing ethanol concentration was used at 4°C.
We employed a polycrystalline sample.
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