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RNAi-targeted downregulation was employed to target Gαi, Gαq, Gαf, and Gαs (Fig. 4A F); of these constructs, those targeting Gαq and Gαi were previously shown efficient in downregulating target gene expression [16], [31].
26 Although there are currently no LSEC-specific Cre drivers, a general endothelial-targeted Cre driver has recently been successfully employed to target LSECs.
A recombineering strategy similar to that successfully used to generate Ehd1 and Ehd4 null mice in our earlier studies [27], [28], was employed to target the 5'untranslated region and the first exon of Ehd3 such that targeted animals would not express EHD3 (Text S1 and Figure S1A).
TPZ was then employed to target the anti-VEGF-induced hypoxic region.
A multiple shooting and continuation procedure is employed to target orbit-attitude periodic solutions in this model.
Systematic process systems engineering tools such as process simulation and pinch analysis are employed to target economically viable production pathways.
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Isoform specific siRNAs employed to selectively target mRNAs isoforms preferentially expressed on cancer cells [ 54] might result in a less toxic cancer therapy.
However, there has been little evidence that bsAb-based targeting strategies can be employed to enhance targeting specificity.
This in turn can be employed to expand targeting space of both current and future Cas9 variants with altered sites restrictions.
A visual oddball paradigm was employed to analyze target processing using event-related potentials.
For example, FPNI-PCR can be employed to generate target genomic products when cell lysates are used to supply the DNA templates.
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