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Since no close homologs of chromosomal passenger proteins other than Aurora B were identified in the T. brucei genome, we employed tandem affinity purification to isolate proteins that form complexes with TbAUK1 in vivo using the PTP (proteinA/tobacco etch virus protease site/proteinC epitope) tagging and purification system, which was recently established in T. brucei [24].
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To identify novel interactors of Glo3, we employed tandem-affinity purification after crosslinking with an HBH-tag followed by LC-MS/MS analysis (Fig. 1A) (Tagwerker et al., 2006).
Therefore, we employed tandems NNC and NNT to reduce the risk of stop codons in the inserted mutants.
In contrast, top-down proteomics employs tandem mass spectrometry to analyze intact proteins.
Using a quantitative proteomic method employing tandem mass spectrometry (MS/MS) and spectral counting, Ueda et al (2010) demonstrated that NAC1 regulated fatty acid synthase (FASN) expression.
The error is minimized by employing Tandem mass spectrometry (MS/MS) that detects the fragmented ions in the spectra, thereby identifying the amino acid modification and peptide identification [ 26].
The application of analytical methods employing tandem mass spectrometry or high resolution mass spectrometry is strongly recommended to confidently identify and accurately quantify BCMs in complex dairy product matrices.
A prototype non-destructive beam-profile monitor employing tandem-type MCPs has been designed and tested at HIMAC.
This suggests the two approaches could be employed in tandem.
A PID feedback of the piston displacement has been employed in tandem for absorbing the unmodeled effects.
These are often employed in tandem to construct stacked Autoencoders [8],[26] and Deep belief networks [7], which are constructed by stacking up Autoencoders and Restricted Boltzmann Machines respectively.
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