Sentence examples for employed restriction from inspiring English sources

We employed restriction digestion and S1 nuclease treatment in conjunction with PFGE and Southern Blot analysis to confirm the sizes and topologies of the plasmids present in JCM1046.

Historically, widely applied methods for the detection of DNA polymorphisms employed restriction enzymes and were limited to detection of changes in the restriction sites.

This study employed restriction enzyme digestion in conjunction with the recombinant cloning of a Norway rat Vegfr2 gene restriction fragment into the eukaryotic expression vector pCMV6.

A synergistic approach of combining libraries created by different methods will help in reducing gaps in the physical map that may result from uneven distribution of restriction sites of the employed restriction endonucleases.

We can get fragment size information by employing restriction enzymes.

A number of these approaches employ restriction endonucleases to discriminate between sequences.

The currently widely used Genome Walker Kit from Clontech employs restriction enzyme cleavage of DNA and adaptor-ligated genomic DNA construction, and also involves a nested PCR strategy.

The first zoning policy employed no restriction while the second conversion restriction zoning policy restricted urban-agricultural expansion in the Guwahati Municipal Development Authority (GMDA) proposed green belt, extending to a third zoning policy providing wetland restoration in the proposed green belt.

To explore the molecular mechanism of this mutagenesis further, we employed the Restriction Fragment Length Polymorphorism (RFLP) method by southern-blot assay.

To test the effect of different anchoring enzymes on the final expression profiles, we employed two restriction enzymes DpnII and BfaI in addition to NlaIII for the isolation of tags from the same cDNA samples.

The performance of barcoding was also compared against the commonly employed NB restriction digest system (also based on COI); while the latter is informative for discriminating gypsy moth subspecies, COI barcode sequences provide greater resolution and generality by encompassing a greater number of haplotypes across all Lymantria species, none shared between species.