While all 15-mer peptides were tested on cells from individual mice, optimal epitope mapping employed pooled samples.
Such challenges notwithstanding, we were able to use the rpart determined ICS prognostic cut-point from the RT-PCR measured node-negative FFPE cases to dichotomize our previously employed pooled microarray datasets of node-negative chemotherapy naive HRneg/Tneg cases into low and high risk subgroups with significant outcome differences.
Due to cost constraints the current approaches for GWAS re-sequencing usually employ pooling strategies.
These results demonstrate that our initial findings employing pools of siRNAs can be reproduced with mono-specific, chemically modified siRNAs.
Our approach still provides an improvement in estimating parental transcription over strategies employing pooled samples, and focusing sampling effort on individual F1s has provided more reliable estimates of both the mean and variance of transcript levels in hybrids.
Contrary to previous studies that supported the validity of RNA sample pooling for microarray based analyses of gene expression [ 21, 26], we documented the pooling bias in estimating differential gene expression, and high false positivity rate to detect DEGs for RNA-seq experiments employing pooling of low amount RNA samples from brain micro-punches.
Key strategies of the main ARV procurement program for PEPFAR to reduce supply chain risks include: (1) employing pooled procurement to reduce procurement and shipping costs and to accommodate changing country needs by making stock adjustments at the regional level, and (2) establishing regional distribution centers to facilitate faster turnaround of orders within defined catchment areas.
The 5-D pooling strategy employing row-pools (RP), column-pools (CP), plate pools (PP), column super-pools (CSP), and row super-pools (RSP) is graphically detailed in Luo et al. 2009 [ 9].
In this research, a questionnaire was employed to pool the real opinions of the experts by an iterative technique of questioning with a consistency analysis.
The random-effects model was employed to pool studies when statistical heterogeneity occurred.
In the case of experiments with mice at 2 and 6 m.o.a., we employed a pool of three five animals for each age group and each genotype.
