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Similar expression and purification protocols were employed for purifying the metavinculin-ΔLeu954 and the His8-tagged truncated mutants metavinculin-ΔH1, metavinculin-ΔH1', and metavinculin-ΔH1ΔH1'.
The former was employed for purifying the Y. lipolytica and P. pastoris enzymes via the interaction of its GST domain with immobilized glutathione and the latter for purifying the P. angusta and S. cerevisiae enzymes.
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We employed protocols for purifying nuclei that are free of ER protein contamination for in vitro analyses and methods for visualizing RNAi factors and RNAi activity in cell nuclei.
In particular, it is unclear whether positive or negative selection is best employed for generating purified cells for profiling.
A HiPrep™ 26/10 column (GE Healthcare) was employed for desalting the purified oligonucleotide using MilliQ™ ultrapure water (Millipore Corporation, Billerica, USA) as eluent.
In the 1880s the Hyatts set up a company that employed a patented process for purifying water through the use of coagulants and filters.
A novel aqueous two-phase system composed of a surfactant and sorbitol was employed for the first time to purify pectinase from Psidium guajava.
Using the same purification scheme as that employed for RBUP, Zhao et al. purified an antitumour lectin AAL from the edible mushroom Agrocybe aegerita, which also possessed DNase activity (Zhao et al. 2003).
The purified erythrocytes were employed for the malaria culture.
To confirm the binding epitope as mapped by the peptide array experiment, a GST-fusion construct containing SKIP residues 43 79 was employed for GST pull-down experiments using purified PPIL1 protein.
Amplicons were purified with ethanol precipitation, and ≥ 3 μg purified products per sample were employed for Illumina GAII library construction and sequencing at Beijing Genomics Institute (Shenzhen, China).
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