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MessageBOOSTER cDNA synthesis amplification kit (Epicentre Biotechnologies, Madison, WI, USA) was employed for prospective validation of gene signatures identified in the microarray on the unpooled individual sorted samples.
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Nor is it employed for egotistic ends.
Other contractors were employed for specialist tasks.
Two techniques were employed for mixing.
Negative controls were employed for each assay.
statistical software were employed for these analyses.
In one prospective cohort study of 36 patients, Menéndez et al. [ 52] showed the greatest predictive value when two predictive scales (PSI and CURB65/CRB65) along with C-reactive protein were employed for the 30-day mortality prediction in CAP.
CORRONA has employed a prospective request for source documentation for these events since 2008.
Acquisition of functional volumes employed Siemens' prospective acquisition correction (PACE) technique for motion correction, in which head movements are calculated by comparing successively acquired volumes and are corrected online [42].
Acquisition of functional volumes employed Siemens' prospective acquisition correction (PACE) technique for motion correction, in which head movements are calculated by comparing successively acquired volumes and are corrected on-line [17], [18].
This study employed a prospective comparative design.
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