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For instance, GC-MS is extensively used for the analysis of semi-polar primary metabolites due to its heat stability, which is applicable for modifications through derivatization reactions that make them volatile [41], whereas LC-MS has been particularly employed for lipid or plant secondary metabolites [66] [68].
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Scheme 1 depicts the bioconjugation approaches that were typically employed for the lipid modification of neuropeptides using appropriate SQ derivatives.
POPC and POPS (or otherwise mentioned) in a ratio of 10 3 were employed for the lipid bilayers in the fluorescence experiments.
Therefore, one strategy that has been employed for quantitation of lipid species across a large number of lipid classes is to include an exogenous lipid molecular species representative of each lipid class of interest.
Therefore, findings of our research suggest that the metabolic engineering strategy adopted in this study for shifting the anabolic carbon flux from starch synthesis to lipid biosynthesis can be employed for increasing the storage lipid content of seeds in other plant species.
Each method currently employed for the determination of lipid content has its advantages and disadvantages, and there is no procedure available which is suitable for all types of lipids.
The technical characteristics of the assays employed for plasma glucose concetrations, lipid profiles and serum hormone measurements have been described elsewhere [6].
Previously, microfluidic CARS flow cytometry has been employed for the enumeration of lipid-rich cells and particles [ 48].
Herewith, we show that ecological principles can effectively be employed for the enrichment of lipid-accumulating species, enabling potential application on a broad variety of waste streams.
Liquid chromatography mass spectrometry (LC-MS) has been successfully employed for the analysis of various lipid classes such as TAGs [ 20– 24] and phospholipids [ 24– 31].
In chemical conversion technologies transesterification process can be employed for the conversion of extracted lipids into biodiesel [ 16].
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