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Summary of the relative expression (in folds) derived from 2-ΔΔ Ct values obtained for various transcripts based on Real Time PCR are given in Table 4. Cb-calibrator The pair of internal primers deduced from pDp1-4 used for expression study was also employed for copy number analysis of these fragments in cattle buffalo, goat and sheep using Real Time PCR.
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The primers standardized and checked for the quantitative expression for JS1 JS12 were employed also for copy number calculation (Additional file 4).
QuMA has been commonly employed for quantitative measurement of DNA copy number [ 21, 36, 37].
In this work, plasmid copy number was not used as an approach to control decoration and a high copy plasmid was employed for all constructs, but this may be of interest in future studies where multiple fusions could be incorporated simultaneously into a phage capsid.
Alternatively, tag-based platforms may be employed for more direct measurement of mRNA copy numbers (Gainetdinov et al., 2007 ; Hu and Polyak, 2006 ).
Assume that the destination chooses selection diversity combining, i.e., when two signals are received at the destination, a signal copy with higher signal-to-noise ratio (SNR) than the other will be employed for decoding the source message.
Therefore, regarding high ribosomal RNA (rRNA) copy numbers in a cell, isothermal nucleic acid sequence-based amplification (NASBA) technique was employed for simple, sensitive and simultaneous detection of the bacteria.
In G-ER, the table-driven routing is employed for the intra-group routing, and ER's strategy with our proposed principle of one inter-group packet copy for one intermediate group is adopted for the inter-group routing.
Further, the level of resistance to antibiotic (2 mg/ml) revealed by different transformants amply testify the integration of 4-7 cofieSAM2 SAM2 gene as these two genes are linked in the recombinant plasmid employed for genetic transformation.
Nor is it employed for egotistic ends.
Other contractors were employed for specialist tasks.
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