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Detection of Binding Sites is employed for binding site detection, when the protein chains meet the requirement of Number of Ligand Atoms and Stability of Complex.
Finally, Detection of Binding Sites will be employed for binding site detection.
The remainder was employed for binding studies (vide infra).
The first bp and the last 3 bp of this motif were deemed to have low information content and removed from further consideration, resulting in a full motif of 12 bp (consensus sequence: AANGTCGTAAAA) being employed for binding site discovery.
Recent crystallographic and biochemical analyses of TDO and IDO have aided our understanding of the mechanisms employed for binding and activating dioxygen by allowing identification of key active-site residues.
Using a semi-quantitative approach similar to that employed for binding of antibodies to other cell surface antigens (Leite et al., 2008; Waters et al., 2008), the binding was scored visually from 0 (no binding) to 4 (very strong binding) by two independent observers.
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The fraction of [S]-Met-tRNAi bound to 40S·eIF1·eIF1A or 40S·eIF1·eIF1A·mRNA complexes was measured using a PhosphorImager, plotted against the 40S subunit concentration, and the data were fit with a hyperbolic or quadratic binding equation, with the latter employed for tight binding.
Different binding site prediction methods were employed for finding the binding site amino acid residues of both monocot and dicot AGPase SS.
At present, several experimental techniques have been employed for identifying DNA-binding proteins, such as filter binding assays, genetic analysis, chromatin immunoprecipitation on microarrays, and X-ray crystallography.
Based on the molecular structure of anle138b, we considered the possibility that this compound has an intrinsic fluorescence that might be employed for direct molecular binding studies.
A takeoff angle of 90° from the surface was employed for each sample and binding energies were calibrated against the Au4f binding energy of the gold surface at 84.0 eV.
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