Employing this platform, we systematically screened four commercially available cationic lipid transfection materials with a range of RNAs encoding the CRISPR-Cas9 system.
In order to demonstrate the generality of our designed strain, we employed this platform for the production of each of 7 different chorismate derivatives.
Employing this platform, we detected 271 differentially expressed (DE) probes according to genetic type, exceeding the threshold PP-value corresponding to a FDR < 0.10 (Posterior Probability =0.006) (Additional file 1).
We have further employed this platform for synthesis of PrA(G,L -PEG-SA adaptors with precisely controlled stoichiometry of 1:1:1 and demonstrated the utility of adaptors for assembly of detection probes for cell staininG,L -PEG-SA, as well as bispecific tarG,L -PEG-SAnds for ligadaptorsen cancer cell lysis by effector T cells.
Further, we have employed this platform for assembly of universal heterobifunctional adaptors consisting of two strong binary affinity systems Protein A(G,L /Antibody and biotin/streptavidin which facilitate simple preparation of antibody antibody, antibody drug, and antibody reporter pairs via self-assembly in a mix-and-use manner.
We employed this vector platform to express hemagglutinin (HA) and neuraminidase (NA) genes from 2009 H1N1 pandemic viruses.
Our prior experience in employing this array platform for cross-species hybridizations indicated that changes as low as 1.2-fold frequently reflected higher changes when assessed by q-PCR ([15]and see q-PCR below).
Indeed, the 2013 Audi A3, introduced at the Geneva motor show in March, was the first production car to employ the platform.
This platform employs purified expression construct DNAs arrayed onto a microscope slide via biotin-avidin interaction.
This platform employs a chip-based TaqMan genotyping technology.
This platform employs an allele-specific primer extension assay using two probes (bead types) in one color channel to score Single-Nucleotide Polymorphisms (SNPs) [ 24, 25].
