Emphasising sensitivity implies a risk of overdiagnosis, which dilutes the actual diagnostic concept, while emphasising specificity implies a risk of underdiagnosis, dismissing patients who might benefit from treatment.
The exclusion of many SCN1A variants considered causal before QC is due to our strict and conservative QC, emphasising specificity above sensitivity.
Importantly, we have shown in the present study that NT5E methylation levels are low in normal melanocytes and benign nevi, emphasising the specificity of NT5E methylation for neoplastic melanocytes.
In addition, protein from commercially available NLL lupin flour and wheat were also tested, demonstrating the proteins remain intact during lupin milling and emphasising the specificity of the antibodies, since no proteins were detected in wheat flour.
The ICAF scores in patients and controls show large differences, both regarding the overall score and each of the four factors, which emphasises the specificity of ICAF for fibromyalgia patients.
Inasmuch as cell debris may bind Ab nonspecifically, it is important to emphasise the specificity controls that were performed: the CFs were not stained by a nonreactive, subclass-matched fluorescent Ab, even in the presence of an unconjugated anti-CD20 Ab, which induces the production of CFs.
Alternative approaches emphasising sensitivity over specificity are possible and these could be assessed using target list cocitations.
Recently, Barrick et al. further emphasised on the specificity of 129S1/SvImJ and C57BL/6J responses to prolonged trans-aortic constriction (TAC), another model of left ventricular hypertrophy associated with increased hemodynamic load and sustained β-adrenergic stimulation [39].
In addition, it is emphasised that the specificity of the HPV analysis increases with age, concomitant with the decreasing prevalence of persistent HPV infections.
Surprising, however, was the difference in positivity between the CLIA (4.1%) and IIF (21.2%) tests in the control group, made up of healthy individuals, thus emphasising the clearly superior specificity of the CLIA assay.
Several papers have reported that these off-target mutations are heritable in humans as well, emphasising the need to improve CRISPR/Cas9 specificity before clinical application.
