Here, we review the application of cryo-ET to the study of eukaryotic cells, with special emphasis on sample preparation, cytoskeleton organization, and macromolecular structures observed at a resolution of 4 6 nm.
Afrocentrism was central, a certain sense of goofy play was common and, especially with Quest, there was an emphasis on sampling jazz rather than the funk records that had formed the backbone of hip-hop since sampling became technically possible, and common, in the late '80s.
By putting major emphasis on sample acquisition, handling, processing, MS signal processing and statistical analyses more robust and reproducible results can be achieved [18], [20], [21].
Current GoMA zooplankton monitoring efforts are likely undersampling the meroplankton due to both the transience of meroplankton production and an emphasis on sampling primarily outside of the near-shore waters where meroplankton are most abundant.
In addition, the MICS manual should put greater emphasis on sampling and, in particular, on the use of probability methods.
In this global bufonid species data set with an emphasis on sampling surrounding the Arabian Peninsula, our analyses consistently place the Arabian toad species within particular genera.
Given the past emphasis on sample size calculations of individual trials that many organizations place on investigators, the number of patients and events should be given much greater consideration in meta-analysis.
The intention of this review is to provide an overview of current methodologies employed in the rapidly developing field of ocular proteomics with emphasis on sample preparation, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS).
US EPA analytical methods for PCBs in water assume that drinking water is being analyzed [ 34, 35] and do not place much emphasis on sampling because the drinking water quality guideline is 0.5 ug/L and only small <1 L samples need to be collected to assess compliance.
