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Emissions scans from 600 to 650 nm were performed using an excitation wavelength of 587 nm.
The scan modes tested were emission scan and synchronous scan Δ60 nm.
We coregistered each emission scan to the corresponding transmission scan and could further confirm that there were no changes in the positioning between transmission and emission scans.
This VOI template was projected onto the entire dynamic emission scan to extract segment TACs.
Emission scan images were reconstructed using a 4.0-mm Colsher filter.
The 60-min emission scan consisted of 20 frames with progressive increase in frame duration.
The samples were excited at 370 nm and emission scan was obtained from 400 to 600 nm.
The ANS binding fluorescence was monitored with emission scan between 400 and 650 nm following an excitation at 350 nm.
As for the patient group, a 45 min dynamic emission scan (3D mode) was initiated with the same frame sequence.
A 20-minute 2D emission scan was performed 30 minutes post-injection and after X-ray based attenuation correction.
Beginning 75 minutes after the injection, an emission scan was performed using the 3D mode, which lasted 15 minutes.
