Exact(1)
An Applied BioSystems 7500 real time PCR system was used with increasing temperature from 5 °C to 94 °C in 1 degree increments, and fluorescence emission was corrected using the control sample without protein.
Similar(59)
This raw emission ratio was corrected for photobleaching and normalized by fitting a single exponential function to the emission ratio trace and dividing the latter by the fitted function; thus all ratio changes were expressed in terms of changes in fluorescence, ΔR/R.
The emission data was corrected for attenuation using the corresponding CT (120 kV/80 mA).
The emission data was corrected in a standardised way (random, scatter and attenuation) and iteratively reconstructed (matrix size, 256 × 256, Fourier rebinning [VIP mode], VUE Point FX [3D] with 3 iterations, 18 subsets).
The change in fluorescence emission of samples titrated with Pif1 was corrected for sample dilution by subtracting the change in the fluorescence emission of oligonucleotide samples titrated with equal volumes of protein sample buffer.
*The passage above was corrected and appended, May 11 , 2011 12 10 ET. A representative of the Fuel Cell & Hydrogen Energy Association referred to fuel cell vehicles as zero emissions vehicles in an interview.
Unfortunately the effect of spot-localized contaminant emission cannot be corrected in commercial scanner microarray data using standard background correction procedures that estimate background signal for a spot from the signal around the spot and thus it leads to errors in the calculated expression ratios (R/G).
Even though the optical density was adjusted by dilution in advance, the fluorescence emission spectra were corrected for "inner-filter effect" [36] according to Zsolnay [21] with a simplified formula that measured fluorescence emission multipliers, e A, where A is the absorbance in cm−1 at the 254-nm excitation wavelength [8, 37].
The effect of turbulence-radiation interaction is approximately taken into account in calculation of radiation emission, which is corrected to allow for temperature self-correlation and absorption-temperature correlation.
The emission data were corrected for decay, dead time, scatter, and random coincidences.
Emission images were corrected for scatter, collimator-detector response, and attenuation using a tidal breathing end-exhale position CT image.
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